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trap staining instructions  (Beijing Solarbio Science)


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    Beijing Solarbio Science trap staining instructions
    In vivo anti-inflammatory effects and inhibition of resorption in subchondral bone. (A and B) <t>Immunofluorescence</t> <t>staining</t> for the iNOS and Arg-1. (C) Micro-CT reconstructed images of subchondral bone tissues at week 4 with various treatments in OA modeled rat joints. (D) <t>TRAP</t> staining for osteoclasts. (E) The proportion of TRAP-stained positive cells. (F and G) Semiquantitative analysis of immunofluorescence staining for iNOS and Arg-1. Results are presented as mean ± SD (n ≥ 4). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Trap Staining Instructions, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+instructions/trap+staining+instructions/pmc12159505-204-9-12
    Average 90 stars, based on 1 article reviews
    trap staining instructions - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Adaptive hydrogel loaded with pre-coordinated stem cells for enhanced osteoarthritis therapy"

    Article Title: Adaptive hydrogel loaded with pre-coordinated stem cells for enhanced osteoarthritis therapy

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2025.05.018

    In vivo anti-inflammatory effects and inhibition of resorption in subchondral bone. (A and B) Immunofluorescence staining for the iNOS and Arg-1. (C) Micro-CT reconstructed images of subchondral bone tissues at week 4 with various treatments in OA modeled rat joints. (D) TRAP staining for osteoclasts. (E) The proportion of TRAP-stained positive cells. (F and G) Semiquantitative analysis of immunofluorescence staining for iNOS and Arg-1. Results are presented as mean ± SD (n ≥ 4). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Figure Legend Snippet: In vivo anti-inflammatory effects and inhibition of resorption in subchondral bone. (A and B) Immunofluorescence staining for the iNOS and Arg-1. (C) Micro-CT reconstructed images of subchondral bone tissues at week 4 with various treatments in OA modeled rat joints. (D) TRAP staining for osteoclasts. (E) The proportion of TRAP-stained positive cells. (F and G) Semiquantitative analysis of immunofluorescence staining for iNOS and Arg-1. Results are presented as mean ± SD (n ≥ 4). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Techniques Used: In Vivo, Inhibition, Immunofluorescence, Staining, Micro-CT

    Mg 2+ and DMOG synergize to inhibit osteoclast activation and modulate the cross-talk between chondrocytes and osteoclasts. (A) CLSM images showing F-actin in bone marrow mononuclear cells (BMMCs) after treatment with osteoclastic medium supplemented with Mg 2+ , DMOG, and M + D for 5 days, respectively. (B) Quantitative assay of TRAP activity at days 3 and 5. (C) TRAP staining of BMMCs. (D to F) Relative expression of mRNA (TRAP, MMP9, and Cathepsin K) of BMMCs. (G) SEM images showing the resorption lacunae formed on bovine dental slices at day 5 with different treatments. (H) Bone resorption area calculated based on G using ImageJ software. (I) Schematic illustration of the suppression of BMMCs differentiation into osteoclasts by Mg 2+ and DMOG. (J) Schematic representation of the interaction between osteoclasts and chondrocytes in Transwell culture. (K) TRAP staining of osteoclasts in the upper chambers. (L) Quantification of TRAP activity expressed by osteoclasts. (M) Analysis of the number of osteoclasts based on the staining of TRAP in K. (N) Staining for ALP, COL-X, and COL-II in chondrocytes located in the lower chambers. (O and P) Based on the relative fluorescence intensity distribution of COL-X and COL-II for the single cell in N. Results are presented as mean ± SD (n ≥ 4). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
    Figure Legend Snippet: Mg 2+ and DMOG synergize to inhibit osteoclast activation and modulate the cross-talk between chondrocytes and osteoclasts. (A) CLSM images showing F-actin in bone marrow mononuclear cells (BMMCs) after treatment with osteoclastic medium supplemented with Mg 2+ , DMOG, and M + D for 5 days, respectively. (B) Quantitative assay of TRAP activity at days 3 and 5. (C) TRAP staining of BMMCs. (D to F) Relative expression of mRNA (TRAP, MMP9, and Cathepsin K) of BMMCs. (G) SEM images showing the resorption lacunae formed on bovine dental slices at day 5 with different treatments. (H) Bone resorption area calculated based on G using ImageJ software. (I) Schematic illustration of the suppression of BMMCs differentiation into osteoclasts by Mg 2+ and DMOG. (J) Schematic representation of the interaction between osteoclasts and chondrocytes in Transwell culture. (K) TRAP staining of osteoclasts in the upper chambers. (L) Quantification of TRAP activity expressed by osteoclasts. (M) Analysis of the number of osteoclasts based on the staining of TRAP in K. (N) Staining for ALP, COL-X, and COL-II in chondrocytes located in the lower chambers. (O and P) Based on the relative fluorescence intensity distribution of COL-X and COL-II for the single cell in N. Results are presented as mean ± SD (n ≥ 4). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Techniques Used: Activation Assay, Activity Assay, Staining, Expressing, Software, Fluorescence

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    Article Title: Reversing the pathological microenvironment by radiocatalytic sensitizer for local orthotopic osteosarcoma radiotherapy enhancement
    Article Snippet: Radiotherapy (RT) can be a means of local control that directly determines the outcome in many special cases of osteosarcoma (OS).. Current strategies focus on radiation-dose amplification with nanosensitizers to increase the radiosensitivity and improve the outcome of OS, but still lack an effective local control strategy for selectively killing the tumor lesions.. Herein, a sandwich-type polyoxotungstate nanocluster (Fe4Se2W18, SWF) with multiple high-Z elements for radiation attenuation and unique electronic structure for a catalytic reaction was designed as a smart nanoradiosensitizer to realize an X-ray-triggered Fenton reaction for enhanced local control of the orthotopic OS.

    Article Title: Adaptive hydrogel loaded with pre-coordinated stem cells for enhanced osteoarthritis therapy
    Article Snippet: .. The procedure for staining is as described in the TRAP staining instructions (Solarbio, China). .. Picrosirius Red was utilized to stain collagen and directionality plugin from ImageJ was used to determine the orientation distribution of the collagen fibers observed in the superficial and deeper zones of articular cartilage.



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    trap staining instructions  (Beijing Solarbio Science)
    90
    Beijing Solarbio Science trap staining instructions
    In vivo anti-inflammatory effects and inhibition of resorption in subchondral bone. (A and B) <t>Immunofluorescence</t> <t>staining</t> for the iNOS and Arg-1. (C) Micro-CT reconstructed images of subchondral bone tissues at week 4 with various treatments in OA modeled rat joints. (D) <t>TRAP</t> staining for osteoclasts. (E) The proportion of TRAP-stained positive cells. (F and G) Semiquantitative analysis of immunofluorescence staining for iNOS and Arg-1. Results are presented as mean ± SD (n ≥ 4). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Trap Staining Instructions, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+instructions/trap+staining+instructions/pmc12159505-204-9-12
    Average 90 stars, based on 1 article reviews
    trap staining instructions - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    trap staining kit according to manufacturer’s instructions  (Fisher Scientific)
    90
    Fisher Scientific trap staining kit according to manufacturer’s instructions
    In vivo anti-inflammatory effects and inhibition of resorption in subchondral bone. (A and B) <t>Immunofluorescence</t> <t>staining</t> for the iNOS and Arg-1. (C) Micro-CT reconstructed images of subchondral bone tissues at week 4 with various treatments in OA modeled rat joints. (D) <t>TRAP</t> staining for osteoclasts. (E) The proportion of TRAP-stained positive cells. (F and G) Semiquantitative analysis of immunofluorescence staining for iNOS and Arg-1. Results are presented as mean ± SD (n ≥ 4). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
    Trap Staining Kit According To Manufacturer’s Instructions, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+staining+instructions/trap+staining+kit+according+to+manufacturer%E2%80%99s+instructions/pm32004923-38-15-9
    Average 90 stars, based on 1 article reviews
    trap staining kit according to manufacturer’s instructions - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    In vivo anti-inflammatory effects and inhibition of resorption in subchondral bone. (A and B) Immunofluorescence staining for the iNOS and Arg-1. (C) Micro-CT reconstructed images of subchondral bone tissues at week 4 with various treatments in OA modeled rat joints. (D) TRAP staining for osteoclasts. (E) The proportion of TRAP-stained positive cells. (F and G) Semiquantitative analysis of immunofluorescence staining for iNOS and Arg-1. Results are presented as mean ± SD (n ≥ 4). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: Adaptive hydrogel loaded with pre-coordinated stem cells for enhanced osteoarthritis therapy

    doi: 10.1016/j.bioactmat.2025.05.018

    Figure Lengend Snippet: In vivo anti-inflammatory effects and inhibition of resorption in subchondral bone. (A and B) Immunofluorescence staining for the iNOS and Arg-1. (C) Micro-CT reconstructed images of subchondral bone tissues at week 4 with various treatments in OA modeled rat joints. (D) TRAP staining for osteoclasts. (E) The proportion of TRAP-stained positive cells. (F and G) Semiquantitative analysis of immunofluorescence staining for iNOS and Arg-1. Results are presented as mean ± SD (n ≥ 4). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: The procedure for staining is as described in the TRAP staining instructions (Solarbio, China).

    Techniques: In Vivo, Inhibition, Immunofluorescence, Staining, Micro-CT

    Mg 2+ and DMOG synergize to inhibit osteoclast activation and modulate the cross-talk between chondrocytes and osteoclasts. (A) CLSM images showing F-actin in bone marrow mononuclear cells (BMMCs) after treatment with osteoclastic medium supplemented with Mg 2+ , DMOG, and M + D for 5 days, respectively. (B) Quantitative assay of TRAP activity at days 3 and 5. (C) TRAP staining of BMMCs. (D to F) Relative expression of mRNA (TRAP, MMP9, and Cathepsin K) of BMMCs. (G) SEM images showing the resorption lacunae formed on bovine dental slices at day 5 with different treatments. (H) Bone resorption area calculated based on G using ImageJ software. (I) Schematic illustration of the suppression of BMMCs differentiation into osteoclasts by Mg 2+ and DMOG. (J) Schematic representation of the interaction between osteoclasts and chondrocytes in Transwell culture. (K) TRAP staining of osteoclasts in the upper chambers. (L) Quantification of TRAP activity expressed by osteoclasts. (M) Analysis of the number of osteoclasts based on the staining of TRAP in K. (N) Staining for ALP, COL-X, and COL-II in chondrocytes located in the lower chambers. (O and P) Based on the relative fluorescence intensity distribution of COL-X and COL-II for the single cell in N. Results are presented as mean ± SD (n ≥ 4). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Journal: Bioactive Materials

    Article Title: Adaptive hydrogel loaded with pre-coordinated stem cells for enhanced osteoarthritis therapy

    doi: 10.1016/j.bioactmat.2025.05.018

    Figure Lengend Snippet: Mg 2+ and DMOG synergize to inhibit osteoclast activation and modulate the cross-talk between chondrocytes and osteoclasts. (A) CLSM images showing F-actin in bone marrow mononuclear cells (BMMCs) after treatment with osteoclastic medium supplemented with Mg 2+ , DMOG, and M + D for 5 days, respectively. (B) Quantitative assay of TRAP activity at days 3 and 5. (C) TRAP staining of BMMCs. (D to F) Relative expression of mRNA (TRAP, MMP9, and Cathepsin K) of BMMCs. (G) SEM images showing the resorption lacunae formed on bovine dental slices at day 5 with different treatments. (H) Bone resorption area calculated based on G using ImageJ software. (I) Schematic illustration of the suppression of BMMCs differentiation into osteoclasts by Mg 2+ and DMOG. (J) Schematic representation of the interaction between osteoclasts and chondrocytes in Transwell culture. (K) TRAP staining of osteoclasts in the upper chambers. (L) Quantification of TRAP activity expressed by osteoclasts. (M) Analysis of the number of osteoclasts based on the staining of TRAP in K. (N) Staining for ALP, COL-X, and COL-II in chondrocytes located in the lower chambers. (O and P) Based on the relative fluorescence intensity distribution of COL-X and COL-II for the single cell in N. Results are presented as mean ± SD (n ≥ 4). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Article Snippet: The procedure for staining is as described in the TRAP staining instructions (Solarbio, China).

    Techniques: Activation Assay, Activity Assay, Staining, Expressing, Software, Fluorescence